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. 2019 Jun 2;16(6):893–901. doi: 10.7150/ijms.27125

Figure 2.

Figure 2

ASC-derived conditioned medium (CM) enhanced apoptosis in MCF-7 and MDA-MB231 breast cancer cells. A) MCF7 and MDA-MB231 cells were cultured with control culture medium or 100% conditioned K-medium (CM) for indicated time points before harvested, and the cell cycle distribution was analyzed using flow cytometry. In each panel, cell cycle profiles are presented together with bar graphs, indicating the cell distribution in each phase of the cell cycle. Presented values represent the mean of three independent experiments ± s.d. *p < 0.05 compared with control. B) Western blot analysis of activated-caspase-3 in MCF-7 or MDA-MB231 cells cultured with 100% ASC-derived CM for 72 hours. C) Western blot analysis of activated-caspase-3 in MCF-7 or MDA-MB231 cells cultured with 50% ASC-derived CM as indicated time points. GAPDH levels are presented as loading controls.