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. 2019 Jun 2;16(6):893–901. doi: 10.7150/ijms.27125

Figure 3.

Figure 3

ASC-derived conditioned medium (CM) induced DNA damage in MCF-7 and MDA-MB231 breast cancer cells. A) MCF-7 and MDA-MB231 cells were cultured with 100% ASC-derived CM for 72 hours before being fixed and stained for DNA content and phosphorylated pSer139 H2AX (γ-H2AX). Images from the immunofluorescence analysis of γ-H2AX positive cells in MCF-7 and MDA-MB231 are presented together with bar graphs, indicating the percentage of the mean of three independent experiments ± s.d. *p < 0.05 compared with control. B) Western blot analysis of γ-H2AX in extracts prepared from MCF-7 and MDA-MB231 cells cultured with 100% ASC-derived CM for 72 h. C) Western blot analysis of γ-H2AX in extracts prepared from MCF-7 and MDA-MB231 cells cultured with 50% ASC-derived CM for indicated time points. GAPDH levels are presented as loading controls.