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. 2019 Jun 2;16(6):893–901. doi: 10.7150/ijms.27125

Figure 4.

Figure 4

ASC-derived conditioned medium (CM) failed to induce apoptosis in M10/H184B5F5 cells. A) M10/H184B5F5 cells were cultured with control culture medium or conditioned K-medium (CM) as a dose-dependent manner for 72 hours before MTT analysis. Presented values represent the mean of three independent experiments ± s.d. *p < 0.05 compared with control. B) M10/H184B5F5 cells were cultured with control culture medium or 100% conditioned K-medium (CM) for indicated time points before harvest, and the cell cycle distribution was analyzed using flow cytometry. In each panel, cell cycle profiles are presented together with bar graphs, indicating the cell distribution in each phase of the cell cycle. Presented values represent the mean of three independent experiments ± s.d. C) Western blot analysis of γ-H2AX and activated- caspase-3 in M10/H184B5F5 cells cultured with ASC-derived CM for indicated time points. GAPDH levels are presented as loading controls.