Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
ASC-derived conditioned medium (CM) induced fully activated ATM-Chk2 cascades in MCF-7 and MDA-MB231, but not in M10/H184B5F5 cells. MCF-7, MDA-MB231, and M10/H184B5F5 cells were cultured with ASC-derived CM for up to 72 hours before the harvest and preparation of cell-free extracts. Phosphorylated pSer1981 ATM and pThr68 Chk2 and the total protein level of Chk2 were analyzed using Western blot. GAPDH levels are presented as loading controls.
