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. 2019 Jun 7;16(7):931–938. doi: 10.7150/ijms.32413

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Optimization of microscale oxygraphy for skin fibroblasts. A-C, Seahorse cell culture plates after 18 hours incubation, 10,000, 25,000 and 65,000 cells/well respectively, confluence is reached for 25,000 cells per well. D, Basal oxygen consumption rate (OCR) in relation to cell number per well. E, Oxygen concentration in relation to cell number per well in maximal oxygen consumption state after FCCP injection. C: closing of the chamber, O: opening of the chamber (re-oxygenation). F, Maximal OCR in relation to FCCP concentration (first and second injection combined).