Role of lncRNA MyH1-AS in skeletal muscle satellite cells differentiation. (A) Expression levels of MyH1-AS in layers and broilers at different developmental stages. (B) Verification of lncRNA MyH1-AS expression levels at 4 developmental stages by qPCR. (C) lncRNA Silencing efficiency. *
P <0.05, ** P <0.01 for comparison between control and silenced group. (D-F) The mRNA expression of MyoD1, MyH3 and MyoG in control and MyH1-AS silenced groups, respectively. *
P <0.05, ** P <0.01 for comparison between control and silencing group. (G-H) The morphological change in myotubes after silencing. (I) The protein expression levels of MyHC and MyoG for comparison between the control and MyH1-AS silenced group, respectively.