Figure 2.

CSE induced NF-κB and NLRP3 inflammasome activation in HUVECs. (A) Expression of protein IκBα and p-IκBα in cell lysates from HUVECs were detected by Western blotting. GAPDH served as the standards. Expression of protein nuclear p65 was detected by cytoplasmic separation Kit. Histone was used as loading control. Quantification analysis of protein p-IκBα (B) and nuclear p65 (C) levels. (D) Nuclear translocation of p65 (red, 400×) was detected by immunofluorescent staining. Expression of protein NLRP3 (E), Caspase-1 (F) and IL-1β (G) was detected by Western blotting. All data were compared with control group and presented as mean ±SEM (n=5 in each group), *P < 0.05, **P < 0.01.