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. 2019 Jun 4;15(8):1591–1599. doi: 10.7150/ijbs.34113

Figure 2.

Figure 2

miR-424-5p inhibits invasion and migration of ICC in vitro. (A & B) Representative images of transwell assays of HuCCT-1 and RBE cells transfected with miR-424-5p mimics or negative control. (C-F) HuCCT-1 and RBE cells were pretreated with NC mimics or miR-424-5p mimics for 24h respectively. Wound-healing assay was performed to explore the motility of each cell line. Similar effect was observed in both HuCCT-1 and RBE cells. G. Cell Counting Kit-8 (CCK-8) assay results show no difference in cell proliferation and cell viability after transfected with NC mimic or miR-424-5p mimic for 24h. H. Representative images of colony formation assay give similar results in cell proliferation. Every experiment above was repeated 3 times independently. *:p<0.05, **:p<0.01, ***:p<0.001 compared to NC group.