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. 2019 Jun 4;15(8):1591–1599. doi: 10.7150/ijbs.34113

Figure 3.

Figure 3

miR-424-5p inhibits ICC metastasis by inhibiting EMT. A. Representative immunofluorescence images of expression of N-cadherin (green signal) in RBE cells after transfected by NC mimic or miR-424-5p mimic for 48h. DAPI (blue signal) was used to counterstain the cell nuclei. B. Representative images of expression of E-cadherin (red signal) in RBE cells after transfected by NC mimic or miR-424-5p mimic for 48h. C. Representative immunofluorescence images of expression of beta-catenin (green signal) in RBE cells. D. The cell lysates of RBE cells transfected with NC mimic or miR-424-5p mimic were used to perform western blot assays with indicated antibodies and GAPDH was used to be the loading control. Every experiment above was repeated 3 times independently.