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. 2019 Jun 4;15(8):1591–1599. doi: 10.7150/ijbs.34113

Figure 5.

Figure 5

ARK5 promotes metastasis of ICC cells in vitro. (A&B) ARK5 knockdown significantly suppressed migration and invasion of HuCCT-1 and RBE cells, as detected by transwell assays. C. Over-expression ARK5 promotes HuCCT-1 cells motility by wound-healing assay. (D&E) Representative immunofluorescence images of the expression of E-cadherin (green signal) and N-cadherin (red signal) in RBE and HuCCT-1 cells after transfected by NC mimic or miR-424-5p mimic for 48h. DAPI (blue signal) was used to counterstain the cell nuclei. As expected, the expression of E-cadherin up-regulated while N-cadherin down-regulating. F. RBE and HuCCT-1 cells were transfected with ARK5 knockdown short hairpin RNA. And the lysates were used to perform western blot analysis with the indicated antibodies and GAPDH was used as a loading control. Error bars represent the S.D. obtained from three independent experiments. *:p<0.05, **p<0.01, ***:p<0.001, ****:p<0.0001.