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CRISPR-Cas9 system mediates CCR5 gene targeting. A: Schematic representation of sgRNAs targeting exon 2 of the CCR5 gene. B: The targeting efficiencies of sgRNA1 and sgRNA2 in MDSC were assessed by the Surveyor nuclease assay. “M” represents the marker, the efficiency of sgRNA1 is 11% and that of sgRNA2 is 28%. C: Plasmid map of Tβ4-knock-in vector. D: Cell line of MDSC, and different stages of monoclonal cells.
