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Physical condition of Tβ4 knock-in goat. A: 9 potential off-target sites predicted by sgRNAcas9. B: Real-time PCR was performed to analyze the expression of CCR5 gene and its adjacent genes in WT, 1702, 1703, and 1704 goats. Error bars represent mean ± SD. * p < 0.05; **p < 0.01. C: Growth curve based on weight of the cashmere goats measured at different periods. D: Blood physiological parameters of four goats.
