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A SCAP was up-regulation in BC cells 5637 and T24 compared to human bladder epithelial immortalized cells SV-HUC1. B, C We knocked down SCAP in 5637 and T24 by using CRISPR-Cas9 in the guidance of two gRNAs, respectively. SCAP expression was significantly downregulated by CRISPR-Cas9 as shown in the figure. Data are shown as mean ± SD (***p< 0.001).
