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. 2019 Jun 10;15(8):1723–1732. doi: 10.7150/ijbs.35284

Figure 4.

Figure 4

Ilamycin E induces apoptosis in HCC1937 and MDA-MB-468 cells (A) Ilamycin E increased Annexin V positive apoptotic cells. HCC1937 and MDA-MB-468 cells were treated with Ilamycin E (30 μM) for 24 to 36 hrs, stained with Annexin V/PI, and analyzed by flow cytometry. (B) The quantitative data of panel A. The percentages of Annexin V-positive cells are shown. *p < 0.05, **p < 0.01. (C) HCC1937 and MDA-MB-468 cells were treated with different concentration of Ilamycin E for 48 hrs. The expression levels of apoptosis related proteins were detected by WB. (D) HCC1937 and MDA-MB-468 cells were treated with 30 μΜ Ilamycin E from 4 to 48 hrs. The expression levels of apoptosis related proteins were detected by WB.