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. 2019 Jun 4;15(8):1676–1684. doi: 10.7150/ijbs.35265

Figure 1.

Figure 1

Combination of oxaliplatin and ALT inhibited the proliferation of colon cancer cells in a cooperative approach. (A-B) HCT116 or RKO cell lines were incubated with increasing doses of oxaliplatin (40-120 μM) and ALT (10 μM). The cytotoxic effect of oxaliplatin and alantolactone was measured by MTT assay. (C-D) The CI values were precisely calculated by the compusyn 2.0 software in a series of FA levels from growth inhibition percentages. A CI < 1.0 was regarded as synergy. (E-H) The induction of apoptosis as assessed by Annexin V/PI staining following combined treatment with oxaliplatin and ALT, and the percentage of apoptotic cells in the treatment groups was calculated. (I-J) HL-7702 or NRK-52E cells were treated with oxaliplatin (80 μM) or ALT (10 μM) alone or their combination (80 μM oxaliplatin and 10 μM ALT) at the indicated doses. At 24 h after treatment, the cell viability was determined by MTT assay. Data represent similar results from three independent experiments (* p< 0.05, ** p < 0.01).