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. 2019 Jun 4;15(8):1676–1684. doi: 10.7150/ijbs.35265

Figure 2.

Figure 2

Elevation in ROS levels was a pivotal event in apoptosis induced by the combination of oxaliplatin and ALT. (A) HCT116 cells were treated with oxaliplatin (60 μM) or ALT (10 μM) alone or their combination (60 μM and 10 μM ALT) at the indicated doses. RKO cells were treated with oxaliplatin (80 μM) or ALT (10 μM) alone or their combination (80 μM oxaliplatin and 10 μM ALT) at the indicated doses. At 2 h after treatment, intracellular ROS levels were measured by flow cytometry. (B) HCT116 or RKO cells were pretreated with 5 mM NAC for 2 h before the combined treatment. At 2 h after treatment, intracellular ROS levels were measured by flow cytometry. (C-F) HCT116 or RKO cells were pretreated with 5 mM NAC for 2 h before exposure to oxaliplatin and ALT combination for 24 h. Percentage of cell apoptosis was determined by flow cytometry. The percentage of apoptotic cells in the treatment groups was calculated. Data represent similar results from three independent experiments (* p< 0.05, ** p < 0.01).