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. 2019 Jun 4;15(8):1676–1684. doi: 10.7150/ijbs.35265

Figure 4.

Figure 4

JNK and p38 MAPK signaling pathways activation is involved in the combined treatment-induced apoptosis of colon cancer cells. (A-B) HCT116 or RKO cells were pretreated with SP600125 for 2 h before exposure to oxaliplatin and ALT combination. The protein levels of p-JNK and JNK were determined by western blot. (C-D) HCT116 or RKO cells were pretreated with BMS-582949 for 2 h before exposure to oxaliplatin and ALT combination. The protein levels of p-p38 and p38 were determined by western blot. (E) HCT116 or RKO cells were pretreated with SP600125 or BMS-582949 for 2 h before exposure to oxaliplatin and ALT combination for 24 h, the cell viability was determined by MTT assay. (F) HCT116 or RKO cells were pretreated with SP600125 or BMS-582949 for 2 h before exposure to oxaliplatin and ALT combination for 24 h, percentage of cell apoptosis was determined by flow cytometry. The percentage of apoptotic cells in the treatment groups was calculated. Data represent similar results from three independent experiments (* p< 0.05, ** p < 0.01).