Fig 6.

Ectopic KLF5 expression sensitizes HCC1806 and HCC1937 cells to JQ-1 and compound 870. A. KLF5 was overexpressed in HCC1806 and HCC1937. The breast cell lines were infected with pCDH-3×Flag-KLF5 or pCDH vector control. The expression of KLF5 was detected by immunoblotting. The upper bands indicate the exogenous 3×Flag-KLF5. The lower bands indicate the endogenous KLF5 protein expression. B. KLF5 overexpression sensitized HCC1806 and HCC1937 cells to JQ-1 and compound 870. pCDH-3×Flag-KLF5 or pCDH vector control lentivirus-infected cells were plated in 96-well plates and treated with JQ-1 for 72 h. The cell inhibition rate was analyzed using the SRB assay. The error bars represent the SD of experimental triplicates.