TABLE 1.
Activity predictions and virulence assays of mutant bacterial strains
| Gene mutated | CAZy and BLAST predictions of gene function | Virulence of mutant on (% tested sites forming tumors)a |
Virulence of the complemented mutant on (% tested sites forming tumors)a |
||
|---|---|---|---|---|---|
| B. daigremontiana | Tomato | B. daigremontiana | Tomato | ||
| bglA (Atu2295) | GH13, α-glucosidase | 0 (0/30) | 0 (0/20) | 30 (3/10) | 25 (2/8) |
| xynA (Atu2371) | GH10, endo-1,4-beta-xylanase | 3 (1/30) | 3 (1/31) | 33 (4/12) | 33 (2/6) |
| arfA (Atu3104) | GH51, arfA, arabinosylfuranosidase | 0 (0/20) | 0 (0/12) | 30 (6/20) | 37 (3/8) |
| pglA (Atu3129) | GH28, pgl, polygalacturonase | 0 (0/43) | 0 (0/19) | 62 (10/16) | 50 (5/10) |
| pglB (Atu4560) | GH28, polygalacturonase | 0 (0/24) | 0 (0/13) | 50 (8/16) | 30 (3/10) |
| bglB (Atu4561) | GH105, rhamnogalacturonyl hydrolase | 0 (0/16) | 0 (0/26) | 25 (4/16) | 25 (2/8) |
| melA (Atu4665) | GH4, melA, α-glucosidase | 0 (0/13) | 0 (0/14) | 30 (3/10) | 27 (4/14) |
a
Number of sites forming tumors/number of sites tested. Virulence of C58 parent was 87% (71/82) on B. daigremontiana and 100% (39/39) on tomato.