TABLE 5.
Properties of glycoside hydrolase genes required for virulence on B. daigremontiana and tomato
| Gene | BLAST and/or CAZy identification | Results of tests of growth on carbon sources | Enzymatic activity | Predicted gene function |
|---|---|---|---|---|
| bglA (Atu2295) | Alpha-glucosidase | Either bglA or bglB is required for growth of A. tumefaciens on lactose or cellobiose, allows growth of E. coli lacZ mutant on lactose | β-Galactosidasea | β-Glycosidase |
| xynA (Atu2371) | Endo-1,4-beta-xylanase | Needed for growth of A. tumefaciens on xylan | NDb | Xylanase |
| arfA (Atu3104) | Arabinosylfuranosidase | Not required for growth on tested carbon sources | Arabinosylfuranosidasec | Arabinosylfuranosidase |
| pglA (Atu3129) | Polygalacturonase | Either pglA or pglB is required for growth of A. tumefaciens on PGA,d allows growth of E. coli on PGA | ND | Polygalacturonase |
| pglB (Atu4560) | Polygalacturonase | ND | Polygalacturonase | |
| bglB (Atu4561) | Rhamnogalacturonyl hydrolase | Either bglA or bglB is required for growth of A. tumefaciens on lactose or cellobiose, allows growth of E. coli lacZ mutant on lactose | β-Galactosidase | β-Glycosidase |
| melA (Atu4665) | MelA, α-galactosidase | Allows growth of E. coli melA mutant on melibiose and on arabinogalactan | ND | α-Galactosidase |
a
Enzyme activity of cloned gene expressed in E. coli.
b
ND, none detected. All cloned genes were tested for β-galactosidase activity.
c
Enzyme activity measured in A. tumefaciens.
d
PGA, polygalacturonic acid.