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. Author manuscript; available in PMC: 2019 Sep 18.
Published in final edited form as: Biochemistry. 2018 Sep 6;57(37):5447–5455. doi: 10.1021/acs.biochem.8b00811

Table 2:

Positional isotope exchange for the α- and β-phosphoryl groups with β-[18O4]-ATP.

Time
(h)		 % α-P
(0,1)		 % α-P (1,0) % β-P
(2,2)		 % β-P
(2,1)		 F ATP
(mM)		 X ʋex
(mM hr−1)		 ʋchem
(mM hr−1)		 ʋex/ʋChem
0 100 0 92 8 5.00
1 86 14 82 18 0.19 4.41 0.11 0.99 0.59 1.68
4 75 25 68 32 0.39 3.91 0.21 0.55 0.27 2.04
8 62 38 58 42 0.57 3.03 0.39 0.41 0.25 1.64
12 51 49 53 47 0.69 2.21 0.55 0.33 0.23 1.43
15 48 52 48 52 0.76 1.66 0.66 0.29 0.22 1.32