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. Author manuscript; available in PMC: 2020 Apr 23.
Published in final edited form as: Biochemistry. 2019 Apr 9;58(16):2144–2151. doi: 10.1021/acs.biochem.9b00189

Figure 1:

Figure 1:

Anion exchange chromatograms for the reaction of MnCTP and phosphoramidate (5). (A) Control sample containing 1.0 mM CTP, 4.0 mM MnCl2, and 10 mM phosphoramidate in 100 mM HEPES/K+, pH 8.0, and 100 mM KCl. (B) Same reaction mixture as in (A), plus the addition of 5.0 μM Cj1416. (C) Authentic cytidine diphosphoramidate standard.