KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Living Colors A.v. Monoclonal GFP Antibody (JL-8)	Clontech Laboratories	Cat # 632381; RRID:AB_2313808
c-Myc (D84C12) Rabbit mAb	Cell Signaling Technology	Cat # 5605S; RRID:AB_1903938
GAPDH rabbit polyclonal Antibody (FL-335)	Santa Cruz Biotecnology	Cat # sc-25778; RRID:AB_10167668
Mouse monoclonal anti-vinculin Antibody	Sigma-Aldrich	Cat # V9131; RRID:AB_477629
anti-p53 ser15 rabbit antibody #9284	Cell Signaling Technology	Cat # 9284s
anti-p21 antibody	Calbiochem/EMD	Cat # OP64
E2F1 antibody	Cell Signaling Technology	Cat # #3742
Chemicals, Peptides, and Recombinant Proteins
Clontech Labs Doxycycline (5g)	Fisher Scientific	Cat # NC0424034
Critical Commercial Assays
GeneJET Genomic DNA Purification Kit	Thermo Fisher Scientific	Cat # K0722
NEBNext Ultra RNA Library Prep Kit for Illumina	NEB	E7530S
RNeasy Plus Mini Kit	QIAGEN	74134
NEBNext Multiplex Oligos for Illumina (Index Primers Set 1)	NEB	E7335S
NEBNext Poly(A) mRNA Magnetic Isolation Module	NEB	E7490S
Deposited Data
Human cancer gene expression data	TCGA	https://tcga-data.nci.nih.gov/docs/publications/tcga
CCLE cancer gene expression data	Cancer Cell Line Encyclopedia	Barretina et al., 2012
SCNA data	TCGA	https://tcga-data.nci.nih.gov/docs/publications/tcga?
RNA-seq	This paper	GSE109326
Experimental Models: Cell Lines
rtTA-HMEC clone 1-9	This paper	N/A
rtTA-HPNE clone 5-12	This paper	N/A
rtTA-IMR90 cells	This paper	N/A
rtTA-MCF7 cells	This paper	N/A
rtTA-HMEC 1-9 p53−/−	This paper	N/A
hTERT-HMECs	Solimini et al., 2012	N/A
hTERT-IMR90s	This paper	N/A
hTERT-HPNEs	Campbell lab	N/A
MCF7s	Polyak lab	N/A
Experimental Models: Organisms/Strains
NSG (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ)	The Jackson Laboratory	Cat # 005557; RRID:IMSR_JAX:005557
Recombinant DNA
Human genome-scale ORF Libraries 1 and 2	This paper	N/A
HMEC ORF sublibrary	This paper	N/A
pInducer-rtTA-Neo	This paper	N/A
pHAGE-TRE-ORF-BC-3′	This paper	N/A
pInducer20-EGFP	This paper	N/A
pX330-U6-Chimeric_BB-CBh-hSpCas9	Cong et al., 2013; Addgene	Plasmid #42230
lentiCRISPR V2 puro vector	Addgene	Plasmid #52961
psPax2	Addgene	Plasmid #12260
pMD2.G	This paper	Plasmid #12259
E2F1sh-1 GGAGTTCATCAGCCTTTCCCCA	This paper	N/A
E2F1sh-2 TAAGAGCAAACAAGGCCCGATC	This paper	N/A
E2F1sh-3 GTGGACTCTTCGGAGAACTTT	This paper	N/A
E2F1sh-4 ATGAGACCTCACTGAATCTGAC	This paper	N/A
OR11L1 -sh (control sh, ctr-sh) ACCACCTGTTGCCTGAAATCAAT	This DaDer	N/A
Sequence-Based Reagents
PCR Primer ORF.BC1.For: CCAGTAGGTCCACTATGAGT	This paper	N/A
PCR Primer ORF.BC1.Rev: CTAGTTCCGCTTACACAGCT	This paper	N/A
PCR Primer ISP-ORF.BC1 .For: GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCCAGTAGGTCCACTATGAGT	This paper	N/A
PCR Primer P5-ORF.BC1.Rev: AATGATACGGC GACCACCGACTAGTT CCGCTTACACAGCT	This paper	N/A
PCR Primer P7-Index-ISP: CAAGCAGAAGACGGCATACGAGATxxxxxxxGTGACTGGAGTT CAGACGTGT	Meyer and Kircher, 2010	N/A
Sequencing Primer heyME19: GCGACCACCGACTAGTTCCGCTTACACAGCT	This paper	N/A
TP53 sgRNA: GTTGCAAACCAGACCTCAGG	This paper	N/A
Software and Algorithms
edgeR	Robinson et al., 2010	http://www.bioconductor.org/packages/release/bioc/html/edgeR.html; RRID:SCR_012802
GSEA	Mootha et al., 2003; Subramanian et al., 2005	http://software.broadinstitute.org/gsea/index.jsp; RRID:SCR_003199
Bowtie 2	Langmead et al., 2009; Langmead and Salzberg 2012	http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
limma	Ritchie et al., 2015	https://bioconductor.org/packages/release/bioc/html/limma.html
Mageck	Li etal., 2014	https://sourceforge.net/projects/mageck/
GISTIC2	Mermel et al., 2011	http://archive.broadinstitute.org/cancer/cga/gistic