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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Epilepsy Res. 2019 May 18;154:132–138. doi: 10.1016/j.eplepsyres.2019.05.006

Figure 1. TTA-P2 ameliorates the hyper-excitability of mEC layer II stellate cells in TLE.

Figure 1.

Action potentials were elicited using depolarizing current steps ranging from −20 to 280 pA. Firing frequency was increased and onset to firing was earlier in TLE (n = 13) compared to control (A; n = 16). Application of the selective T-type Ca2+ channel blocker, TTA-P2 (1 μM), reduced firing rates of control (n = 7) and TLE (n = 8) neurons to similar levels (B and C). Representative traces for control (D) and TLE (E) before and after application of TTA-P2, at current injection steps of 60, 140, 220, and 280pA. Action potentials were also evoked synaptically by stimulation of mEC layer III neurons. Representative traces for control (F) and TLE (G) before and after application of TTA-P2 (1 μM). Application of TTA-P2 reduced evoked action potentials in TLE neurons but had no effect on control neurons (right hand side F and G). Values represent means ± S.E.M. * P<0.05 (Students t-test or one-way ANOVA).