KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal (D3H8P) Proliferating cell nuclear antigen (PCNA) | Cell Signaling Technologies | 13110 |
| Rabbit monoclonal (Asp175) Cleaved-caspase-3 | Cell Signaling Technologies | 9664 |
| Rabbit polyclonal Myeloperoxidase (MPO) | Agilent Technologies | A039829-2 |
| Rabbit monoclonal (C83B10) Acetyl CoA Carboxylase (ACC) | Cell Signaling Technology | 3676 |
| Rabbit polyclonal phospho-Acetyl CoA Carboxylase (Ser79/221) | Cell Signaling Technology | 3661 |
| Rabbit polyclonal AMP-activated protein kinase (AMPK) | Cell Signaling Technologies | 2532 |
| Rabbit monoclonal (40H9) phospho-AMPK (Thr172) | Cell Signaling Technologies | 2535 |
| Rabbit polyclonal Fatty acid synthase (FASN) | Cell Signaling Technologies | 3189 |
| Rabbit polyclonal ATP-citrate lyase (ACLY) | Cell Signaling Technologies | 4332 |
| Rabbit polyclonal beta-Actin | Cell Signaling Technologies | 4967 |
| Rabbit monoclonal (D16H11) Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Cell Signaling Technologies | 5174 |
| Rabbit monoclonal (D8H5) UNC-51-like kinase 1 (ULK1) | Cell Signaling Technologies | 8054 |
| Rabbit monoclonal (D1H4) phospho-ULK1 (Ser555) | Cell Signaling Technologies | 5869 |
| Bacterial and Virus Strains | ||
| None | ||
| Biological Samples | ||
| None | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Diethylnitrosamine | Sigma | N-0756 |
| ND-654 | Nimbus/Gilead | |
| Sorafenib | Selleck | S1040 |
| Phenformin | Sigma | P7045 |
| Critical Commercial Assays | ||
| Proteome Profiler™ Array, Human Phospho-Kinase Array Kit | R&D Systems | ARY003B |
| OxiSelect™ Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation) | Cell Biolabs | STA-320 |
| Deposited Data | ||
| None | ||
| Experimental Models: Cell Lines | ||
| HepG2 | ATCC | HB-8065 |
| Experimental Models: Organisms/Strains | ||
| Wistar rats | Charles River Laboratories | Strain 003 |
| Acc1–S79A KI and Acc2–S212A KI mice | OzGene Pty Ltd | |
| Oligonucleotides | ||
| 18S | Life Technologies | Hs03003631_g1 |
| Cxcl1 | Life Technologies | Rn00578225_m1 |
| Il-1b | Life Technologies | Rn00580432_m1 |
| Il-6 | Life Technologies | Rn01410330_m1 |
| Tnfa | Life Technologies | Rn99999017_m1 |
| Cd80 | Life Technologies | Rn00709368_m1 |
| Cd163 | Life Technologies | Rn01492519_m1 |
| Tbp | Life Technologies | Mm01277042_m1 |
| Il-6 | Life Technologies | Mm00446190_m1 |
| Tnfa | Life Technologies | Mm00443258_m1 |
| Timp1 | Life Technologies | Mm01341361_m1 |
| Col1a1 | Life Technologies | Mm00801666_g1 |
| Col4a1 | Life Technologies | Mm01210125_m1 |
| Acta2 | Life Technologies | Mm00725412_s1 |
| Recombinant DNA | ||
| pGS –gRNA-Cas9-PuroGeneCRISPR | GenScript | |
| Software and Algorithms | ||
| Excel | Microsoft | |
| Prism Version 5.02 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| Nearest Template Prediction | ||
| NIS-Elements Software | Nikon | |
| Other | ||