Table 1.
Coefficients of variation (CV, %) for each miRNA and method evaluated.
| miRNA | PAR | INV | NOR | |||||
|---|---|---|---|---|---|---|---|---|
| N | CV (%) | N | CV (%) | N | CV (%) | |||
| cel-miR-39 | 10 | 69.5 | 10 | 15.3 | ||||
| hsa-miR-21-5p | 10 | 39.1 | 10 | 22.2 | 10 | 30.5 | ||
| hsa-miR-451a | 10 | 35.4 | 10 | 24.0 | 10 | 24.2 | ||
| hsa-miR-92a-3p | 10 | 36.6 | 10 | 27.4 | 10 | 30.8 | ||
| hsa-miR-22-3p | 10 | 52.7 | 10 | 21.9 | 10 | 13.9 | ||
| hsa-miR-1911-5p | ||||||||
| hsa-miR-1264 | ||||||||
| hsa-miR-30c-5p | 10 | 38.7 | 10 | 39.1 | ||||
| hsa-miR-34b-3p | ||||||||
| Mean CV (%) | 40.5 ± 7.0 | 33.0 ± 20.5 | 25.6 ± 9.8 | |||||
NOR had the lowest coefficient of variation, followed by the INV and PAR methods. Only the cases in which the miRNAs were detected in all 10 replicates were considered. Abbreviations: number of replicates analyzed (N), mirVana PARIS Kit from Ambion (PAR), Total Exosome Isolation Reagent from Invitrogen (INV), and Plasma/Serum RNA Purification Kit from Norgen (NOR).