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. 2019 Jul 9;9(16):4764–4778. doi: 10.7150/thno.33139

Figure 4.

Figure 4

The ESCMe reverses tumor phenotype by inhibiting the PI3K pathway. (A) Expression of PI3K pathway genes in C918 cells from the Ctrl, TCo, TCo +VO, CCo, and CCo+VO groups, as assessed by RT-qPCR (n = 3 biological repeats). Delta CT means of genes in C918 cells were shown in Table S3. (B) Expression of upstream activators and suppressors of the PI3K pathway in C918 cells from the Ctrl, TCo, and CCo groups, as assessed by RT-qPCR (n = 3 biological repeats). Delta CT means of genes in C918 cells were shown in Table S4. (C) Western blotting of AKT1/2/3 in C918 cells from the Ctrl, TCo, TCo +VO, CCo, and CCo+VO groups. β-actin served as the internal control. (D) Immunofluorescence assays of AKT1/2/3 in C918 cells. Scale bar, 50 μm. Data are means ± SEMs. *P< 0.05; **P< 0.01; ***P< 0.001; ****P< 0.0001.