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. 2019 Jul 9;9(16):4764–4778. doi: 10.7150/thno.33139

Figure 5.

Figure 5

The ESCMe can suppress the aggressive phenotype of tumor cells while preventing the senescence of normal somatic cells in vitro. (A) Proliferation of RPE and C918 cells sorted from C918 cells co-cultured with RPE cells (Ctrl) and from C918 cells co-cultured with RPE cells and ESCs (ESC), as assessed by CCK8 proliferation assay (n = 4 biological repeats). (B) Cell cycle distribution of RPE and C918 cells sorted from the Ctrl and ESC groups, as assessed by flow cytometry (n = 4 biological repeats). (C) Percentages of apoptotic RPE and C918 cells sorted from the Ctrl and ESC groups, as assessed by flow cytometry (n = 3 biological repeats). (D) Numbers and representative images of clones formed by RPE and C918 cells (n = 3 biological repeats). Delta CT means of genes in RPE and C918 cells were shown in Table S5. (E) Fold change of RNA expression in RPE and C918 cells from the ESC group compared with those in the Ctrl group (n = 3 biological repeats). (F) Immunofluorescence assays of AKT1/2/3 in RPE and C918 cells from the Ctrl and ESC groups. Scale bar, 50 μm. (G) Western blotting of RPE and C918 cells from the Ctrl and ESC groups. β-actin served as the internal control. Data are means ± SEMs. *P< 0.05; **P< 0.01; ***P< 0.001; ****P< 0.0001.