Table 1. Multiple Reaction Monitoring (MRM) Conditions of 22 Glucosinolates in HILIC–MS/MS Analysisa.
| glucosinolates | RT (min) | precursor ion | product ion | CE (V) | internal standard | calibration curve |
|---|---|---|---|---|---|---|
| aliphatic | ||||||
| sinigrinb | 3.94 | 358.0 | 97, 195 | 24 | glucosinalbin | sinigrin |
| glucoiberinb | 11.30 | 422.0 | 259, 358 | 26 | glucosinalbin | glucoiberin |
| glucocheirolinb | 5.19 | 438.0 | 97, 259 | 26 | glucosinalbin | glucocheirolin |
| glucoraphasatin | 2.79 | 418.0 | 97, 259 | 26 | glucosinalbin | glucoerucin |
| glucosativin | 3.45 | 406.0 | 97, 259 | 26 | glucosinalbin | glucoerucin |
| glucoerucinb | 3.00 | 420.0 | 97, 259 | 26 | glucosinalbin | glucoerucin |
| glucorapheninb | 10.30 | 434.0 | 97, 259 | 24 | glucosinalbin | glucoraphenin |
| glucoraphaninb | 11.20 | 436.0 | 97, 372 | 26 | glucosinalbin | glucoraphanin |
| gluconapinb | 3.60 | 372.0 | 97, 259 | 26 | glucosinalbin | gluconapin |
| epiprogoitrinb | 5.42 | 388.0 | 97, 259 | 24 | glucosinalbin | epiprogoitrin |
| progoitrinb | 5.72 | 388.0 | 97, 259 | 24 | glucosinalbin | progoitrin |
| glucoalyssinb | 10.46 | 450.1 | 97, 259 | 26 | glucosinalbin | glucoalyssin |
| glucobrassicanapinb | 3.10 | 386.1 | 97, 259 | 26 | glucosinalbin | glucobrassicanapin |
| gluconapoleiferin | 5.00 | 402.1 | 97, 259 | 26 | glucosinalbin | glucobrassicanapin |
| indole | ||||||
| glucobrassicinb | 3.23 | 447.1 | 97, 259 | 26 | glucosinalbin | glucobrassicin |
| neoglucobrassicin | 2.21 | 477.1 | 97, 259 | 24 | glucosinalbin | glucobrassicin |
| 4-methoxyglucobrassicin | 3.29 | 477.1 | 97, 259 | 26 | glucosinalbin | glucobrassicin |
| 4-hydroxyglucobrassicinb | 4.52 | 463.0 | 97, 259 | 26 | glucosinalbin | 4-hydroxyglucobrassicin |
| aromatic | ||||||
| glucotropaeolinb | 2.65 | 408.0 | 97, 259 | 24 | glucosinalbin | glucotropaeolin |
| gluconasturtiinb | 2.49 | 422.1 | 97, 259 | 24 | glucosinalbin | gluconasturtiin |
| glucobarbarinb | 10.96 | 438.1 | 97, 259 | 26 | glucosinalbin | glucobarbarin |
| glucosinalbinb,c | 4.30 | 424.0 | 97, 259 | 26 | glucoraphenin | glucosinalbin |
a
RT, retention time; CE, collision energy.
b
These 17 glucosinolates have available standards.
c
Glucosinalbin is present only in rocket salad and does not exist in other 11 examined vegetables. In this study, it was used as an internal standard for quantitative analysis of glucosinolates in the 12 vegetables, except rocket salad, in which glucoraphenin was used as the internal standard for quantitative analysis of glucosinolates, as it is absent in rocket salad.