Table 2. Method Validation of HILIC–MS/MS for 17 Glucosinolates with Available Standards^a.

	calibration curve					accuracy (%)		recovery (%)
glucosinolates	formulation	R2	concentration (ng/mL)	LOD (μg/g dry weight)	LOQ	intrabatch	interbatch	low	high
sinigrin	y = 0.037x – 0.106	0.9997	1–500	0.007	0.023	4.35	5.96	79.44	103.06
glucoiberin	y = 0.006x + 0.012	0.9994	1–500	0.003	0.010	4.82	6.32	112.10	84.36
glucocheirolin	y = 0.038x – 0.036	0.9998	1–500	0.004	0.013	4.17	3.34	95.74	106.37
glucoerucin	y = 0.073x – 0.725	0.9991	1–1000	0.009	0.030	7.21	6.76	84.04	110.19
glucoraphenin	y = 0.47x – 1.149	0.9985	1–500	0.001	0.003	2.00	8.85	90.45	84.25
glucoraphanin	y = 0.018x – 0.020	0.9998	1–500	0.013	0.043	4.06	7.67	85.26	98.40
gluconapin	y = 0.054x – 0.012	0.9999	1–250	0.003	0.010	8.61	5.96	78.36	87.43
epiprogoitrin	y = 0.033x – 0.068	0.9994	1–250	0.007	0.023	6.10	5.70	117.01	96.59
progoitrin	y = 0.015x – 0.061	0.9997	1–1000	0.028	0.093	4.23	5.35	84.69	95.64
glucoalyssin	y = 0.017x + 0.029	0.9991	1–500	0.013	0.043	6.74	9.04	95.26	114.17
glucobrassicanapin	y = 0.079x – 0.936	0.9987	1–1000	0.008	0.027	9.24	9.95	81.67	91.45
glucobrassicin	y = 0.042x – 0.133	0.9995	1–500	0.019	0.063	5.51	8.75	92.30	120.31
4-hydroxyglucobrassicin	y = 0.004x – 0.016	0.9996	1–1000	0.021	0.070	2.43	9.02	76.46	107.01
glucotropaeolin	y = 0.082x – 0.265	0.9998	1–500	0.004	0.013	5.58	7.29	120.14	106.33
gluconasturtiin	y = 0.052x – 0.129	0.9990	1–500	0.006	0.020	4.29	7.16	105.76	102.56
glucobarbarin	y = 0.002x + 0.003	0.9979	5–500	0.023	0.077	6.54	3.33	114.79	99.05
glucosinalbin	y = 0.003x + 0.008	0.9991	5–500	0.007	0.023	2.02	8.99	95.01	101.22

^aLOD, limit of detection; LOQ, limit of quantification.