Figure 7.

Extra RFP foci phenotype. Plasmids encoding either Spc42-GBP-RFP or Spc42-RFP were directly transformed into the GFP strains identified in the screen for SPB number aberrations and imaged. A: Representative images of Bbp1, Nsp1, Pom34 and YPR071W -GFP strains expressing Spc42-GBP-RFP from a plasmid, each showing more than two RFP foci, interpreted as indicative of overduplication of SPBs. All scale bars are $5\mu$m. B: Quantification of the microscopy analysis, with key proteins highlighted. Three images were captured for each strain and the percentage of living cells showing more than two RFP foci was calculated. Error bars show $95\text{\%}$ confidence intervals calculated with the Clopper-Pearson exact method; p-values were calculated using Fisher’s exact test. C: Multipolar spindle morphology in the Spc42-GBP-RFP Bbp1-YFP strain, the spindle is labeled by mTurq-Tub1. Green lines indicate the observed spindle morphology, from the mTurq-Tub1 signal, relative to the cell morphology observed in DIC. Scale bars are $5\mu$m.