Fig. 4.

Niclosamide (Niclo) induced degradation of Fzd1-GFP is reversed by autophagy inhibitor 3MA. (A) U2OS cells stably expressing Fzd1-GFP were treated with the indicated reagents for 12h and lysed with the SDS sample buffer for Western blot analysis. MG132 alone inhibited the Fzd1-GFP degradation through protein ubiquitination and resulted in multiple bands. (B) Quantification of the Western blots. Multiple bands resulted from each of the treatments shown in (A) were quantified by normalizing to β-actin and compared using one-way ANOVA. Results are mean ± SEM, n = 3, a: P<0.0001 of DMSO vs Niclo, b: P<0.0001 of DMSO vs MG132, c: P=0.009 of Niclo vs MG132 + Niclo, d: P=0.9801 of DMSO vs 3MA, e: P=0.009 of Niclo vs 3MA + Niclo.