Table 3.

Conversion of CPF to CPO and TCP in Culture

Exposure Conditions		CPF Added		CPF Measured		CPO Measured		TCP Measured
				Mean ± S.E.M		Mean ± S.E.M		Mean ± S.E.M
CPF Exposed Neurons (1 hr)		100 nM		2.22 ± 0.11 ng		ND		<LLOQ
		1000 nM		25.05 ± 0.57 ng		<LLOQ		<LLOQ
		10000 nM		180.57 ± 0.84 ng		0.01 ± 0.00 ng		2.69 ± 0.02 ng
CPF Exposed Neurons (24 hr)		100 nM		0.47 ± 0.00 ng		ND		ND
		1000 nM		3.59 ± 0.11 ng		0.01 ± 0.00 ng		ND
		10000 nM		39.95 ± 2.66 ng		0.03 ± 0.00 ng		0.59 ± 0.13 ng
Media From CPF Exposed Neurons (1 hr)		100 nM		67.77 ± 2.70 nM		ND		<LLOQ
		1000 nM		611.36 ± 32.04 nM		<LLOQ		<LLOQ
		10000 nM		4759.43 ± 70.72 nM		0.57 ± 0.02 nM		<LLOQ
Media From CPF Exposed Neurons (24 hr)		100 nM		13.68 ± 0.23 nM		ND		ND
		1000 nM		118.01 ± 7.26 nM		<LLOQ		ND
		10000 nM		2053.49 ± 68.24 nM		0.39 ± 0.06 nM		1224.68 ± 52.39 nM
CPF Exposed Media (no neurons present)		100 nM		ND		ND		ND
(24 hr)		1000 nM		191.62 ± 26.42 nM		ND		ND
		10000 nM		2449.84 ± 111.67 nM		0.57 ± 0.06 nM		829.37 ± 113.13 nM

CPF = chlorpyrifos; CPO = chlorpyrifos oxon; TCP = 3,5,6-trichloropyridinol; ND = Not Detected; LLOQ = lower limit of quantification

Each value represents the mean ± s.e.m obtained from 4–6 replicants per toxin concentration obtained in 2 separate experiments