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. Author manuscript; available in PMC: 2019 Jul 22.
Published in final edited form as: Nanoscale. 2018 Mar 15;10(11):5078–5088. doi: 10.1039/c7nr06779h

Fig. 4.

Fig. 4

Uptake of BCNs and effect on cell viability. (a) MTT assay of RAW 264.7 macrophages incubated with BCNs for 24 or 48 hours at different concentrations. Error bars represent SD, n = 3. (b) Flow cytometric analysis of BCN uptake by RAW macrophages at different timepoints of incubation, error bars represent SD, n = 3. (c) Confocal images of macrophages stained with lysosomal dye Lysotracker (green) and DNA dye DAPI (blue) following incubation with Texas Red labeled BCNs (red) for 2, 4, or 8 h. White arrows in the merged image point to examples of colocalization of Lysotracker and Texas Red signals, demonstrating endolysosomal uptake of BCNs.