FIGURE 1.

Analysis of P2Y₂R expression or P2Y₂R function in submandibular gland (SMG), spleen, and isolated SMG epithelial and B cells from IL-14αTG and wild-type mice. The RT-PCR analysis was performed with cDNA prepared from (a) whole SMG and (b) the whole spleen from 9-month-old wild-type (WT), IL-14αTG, and IL-14αTG × P2Y₂R^−/− mice. Data represent means ± SEM for SMGs from wild-type (n = 5), IL-14αTG (n = 8), and IL-14αTG × P2Y₂R^−/− (n = 6) mice and spleens from wild-type (n = 3), IL-14αTG (n = 4), and IL-14αTG × P2Y₂R^−/− (n = 6) mice, where * indicates p < .05. To determine functional P2Y₂R expression, changes in [Ca²⁺]_i in response to the P2Y₂R agonist UTP (100 μ mol L⁻¹) were measured in freshly isolated (c) epithelial cells from SMGs and (d) B cells from the spleens or SMGs of 12-month-old IL-14αTG and wild-type control mice. Data are means ± SEM of UTP-induced Δ[Ca²⁺]_i in pooled SMG epithelial cells from five wild-type or IL-14αTG mice or pooled splenic or SMG B cells from nine wild-type or three IL-14αTG mice