FIGURE 3.

Lymphocytic foci in the submandibular gland (SMG) of IL-14αTG and IL-14αTG × P2Y₂R^−/− mice are composed of B and T cells. (a) Serial 5-μm sections of paraffin-embedded SMGs from 9-month-old IL-14αTG and IL-14αTG × P2Y₂R^−/− mice were subjected to immunostaining for the B cell marker B220 (top panels), the T cell marker CD3 (middle panels) or hematoxylin and eosin staining (bottom panels). Images are representative of similar results for n = 3 mice for each genotype; scale bar = 100 μm. cDNA derived from whole SMGs of 9-month-old mice was utilized for RT-PCR analysis to determine mRNA expression of (b) the B cell marker CD20 and (c) the T cell marker CD3. Data represent means ± SEM for SMGs of wild-type (n = 5), IL-14αTG (n = 7), P2Y₂R^−/− (n = 7), and IL-14αTG × P2Y₂R^−/− (n = 20) mice, where ** indicates p < .01