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. Author manuscript; available in PMC: 2019 Jul 22.

Published in final edited form as: Int J Radiat Oncol Biol Phys. 2016 Jan 22;95(2):782–790. doi: 10.1016/j.ijrobp.2016.01.028

Figure 3. — Effects of AZD1775 on radiation induced DNA damage repair. HCC cell lines were treated with AZD1775 for 1 hour then irradiated with 6 Gy. At the indicated time points, cells were fixed and stained with anti-γH2AX antibody and PI then analyzed by flow cytometry. A, scatter plots at 16 hours after radiation therapy. Combined treatment with AZD1775 followed by radiation resulted in more γH2AX positive cells at 16 hours compared to radiation alone. Additionally, the intensity of γH2AX staining was greater with the combination of RT and AZD1775 in all cell lines. B, Time course of γH2AX positivity after radiation therapy for each cell line.

Figure 3. — Effects of AZD1775 on radiation induced DNA damage repair. HCC cell lines were treated with AZD1775 for 1 hour then irradiated with 6 Gy. At the indicated time points, cells were fixed and stained with anti-γH2AX antibody and PI then analyzed by flow cytometry. A, scatter plots at 16 hours after radiation therapy. Combined treatment with AZD1775 followed by radiation resulted in more γH2AX positive cells at 16 hours compared to radiation alone. Additionally, the intensity of γH2AX staining was greater with the combination of RT and AZD1775 in all cell lines. B, Time course of γH2AX positivity after radiation therapy for each cell line.