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. 2019 Jul 16;10:1631. doi: 10.3389/fimmu.2019.01631

Figure 5.

Figure 5

Expression level of NLRP3, NLRP6, NLRP12, IFI16 and AIM2 inflammasomes in human primary corneal epithelial cells infected with virulent and less-virulent strains of HSV-1. Human primary corneal epithelial cells (hTCEpi cell line) were infected in vitro with HSV-1 laboratory strains McKrae, 17, F, RE or KOS (A,B) or with clinical isolates KOS79 and KOS63 (C,D) at MOI of 1. Two, 8 and 24 h post-infection, hTCEpi cells were harvested and immunoblots of whole cell lysates were performed for expression of NLRP3, NLRP6, NLRP12, IFI16, and AIM2 inflammasomes. Corresponding β-actin was used as a control. (B,D) Graphs show the kinetics of fold changes in NLRP3, NLRP6, NLRP12, IFI16, and AIM2 inflammasomes expression at 2, 8, and 24 h post-infection with virulent/less-virulent strains of HSV-1 compared to the control and normalized to β-actin. Results are representative of three experiments.