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. 2019 Apr 15;28(15):2549–2560. doi: 10.1093/hmg/ddz078

Figure 4.

Figure 4

NRAP inhibits KLHL40-NEB interaction. KLHL40 and NRAP or nebulin were overexpressed in Klhl41 C2C12 myoblasts. (A) Co-expression of KLHL40 and N-terminal nebulin (N-NEB) or NRAP in C2C12 myoblasts revealed protein–protein interactions between KLHL40/N-NEB or KLHL40-NRAP. In the presence of nebulin, KLHL40 demonstrated preferential binding to NRAP. (B) Similarly, overexpression in C2C12 and Western blot analysis revealed that KLHL40 interacts with C-terminal nebulin (C-NEB), and this interaction is blocked in the presence of NRAP.