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. 2019 Jul 15;9:253. doi: 10.3389/fcimb.2019.00253

Figure 1.

Figure 1

FOM inhibits the hemolytic activity of Hla in vitro. (A) S. aureus 8325-4 were incubated in TSB at 37°C to a post-exponential growth phase (OD 600nm = 2.5) with or without FOM. The supernatant protein was collected, proteins were tested by western blot analysis. (B) Colony counts of S. aureus 8325-4 treated with different concentrations of FOM. (C) Hemolytic ability of S. aureus 8325-4 supernatant assay. (a: TSB; b: 2 MIC; c: 1 MIC, d: 2−1 MIC; e: 4−1 MIC; f: 8−1 MIC; g: 16−1 MIC; h: Supernatant of S. aureus 8325-4). (D) Hemolytic ability of S. aureus 8325-4 supernatant assay with the red cells of rabbit co-incubation. (E) Supernatant of S. aureus 8325-4 hemolytic ability assay by measuring the absorbance at 450 nm. (F) Hemolytic ability of α-Hla and SMVs and with or without FOM assay. (a: TSB; b: α-Hla; c: α-Hla ± FOM, d: SMVs; e: SMVs ± FOM). Data are the means ± standard errors derived from three experiments. * P < 0.05, ** p < 0.01, *** p < 0.001, significantly different from S. aureus 8325-4.