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. 2019 Jul 15;9:253. doi: 10.3389/fcimb.2019.00253

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Copyright © 2019 An, Wang, Zhan, Tang, Shen, Shen, Wang, Luan, Wang, Wang, Liu, Zheng and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MAPKs mediated the activation of NLRP3 inflammasome in Mϕ. (A) Western blot was used to test the activation of NLRP3 inflammasomes-related proteins (ASC, caspase-1 p20, Pro-caspase-1, IL-1β, Pro-IL-1β, IL-18, Pro-IL-18 and NLRP3) in Mϕ with ERK inhibitor (PD98059), p38 inhibitor (SB203580), and JNK inhibitor (SP600125) in the both culture supernatant or cell lysate. (B,C) Quantitative detection of IL-18 and IL-1β secretion by ELISA. (D) The activation of NLRP3 inflammasomes (ASC, caspase-1 p20, Pro-caspase-1, IL-1β, Pro-IL-1β, IL-18, Pro-IL-18, and NLRP3) in the both culture supernatant and cell lysate in Mϕ were analysis by western blot and three inhibitors were used. (E,F) Quantitative detection of IL-18 and IL-1β secretion by ELISA. Sup is culture supernatant and cell are cell lysate. Data are means ± standard errors derived from three experiments. * P < 0.05, ** p < 0.01, *** p < 0.0001.