Figure 3.

Flow cytometry 2D density plots (FSC vs SSC) of 15 μM rSpSM30B/C-G and 1.5 μM rSpSM50 proteins in 10 mM HEPES, pH 8.0; 10 mM HEPES/30 mM NaCl, pH 8.0; and 8.9 mM NaH₂PO₄, pH 4.0. FSC refers to a parameter measuring light scattered less than 10° as a particle passes through the laser beam and is related to the particle size. SSC is proportional to particle granularity or internal complexity and is a measurement of mostly refracted and reflected light that occurs at any interface within the particle where there is a change in RI. SSC is collected at approximately 90° to the laser beam by a collection lens and then redirected by a beam splitter to the appropriate detector.