Fig. 3. Encoding strategy for short single-stranded DNA conjugates based on a DNA-hairpin. (A) Gel analysis of the ligation of the DNA-hairpin and DNA-isoquinolone conjugate (sequences of DNAs, hairpin: 3′-CAA ATC CGT TCA S AGG TCG GTG TGA ACG GAT TTG AGT C-5′, code 1: 3′-TCC ATT ACG CT(alkyne)C TC-5′, counter strand 1: 3′-TCA GGA GAA ATG GAT GGA CAT A-5′, S = C6-Spacer). (B) Scheme of a proposed split and pool DNA-encoded library synthesis for the Castagnoli–Cushman reaction based on the hairpin encoding strategy. AMA = 30% aqueous ammonia/40% aqueous methylamine, 1 : 1 (vol/vol).
