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. 2019 Jul 15;10:891. doi: 10.3389/fphys.2019.00891

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Copyright © 2019 Kotwica-Rolinska, Chodakova, Chvalova, Kristofova, Fenclova, Provaznik, Bertolutti, Wu and Dolezel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Examples of the in vivo sgRNA efficiency test. Pictures of polyacrylamide gels showing the heteroduplex mobility assay on eggs injected with different sgRNAs targeting pdf gene. The name of the particular sgRNA is mentioned above picture of the gel. M, marker; WT, wild type, single eggs screens are marked by numbers. Additional bands indicate high frequency of mosaicism in the egg (for example egg 1 in PDF 2 gel). Black arrow points to the nonspecific band occurring also in WT eggs and should not be mistaken for occurrence of mosaicism.