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. 2019 Jul 15;10:891. doi: 10.3389/fphys.2019.00891

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Copyright © 2019 Kotwica-Rolinska, Chodakova, Chvalova, Kristofova, Fenclova, Provaznik, Bertolutti, Wu and Dolezel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Guide RNAs on-target efficiency. (A) Mutagenesis efficiency (% of G₀ parents producing F₁ heterozygotes of the total fertile G₀ parents) is positively correlated to the percentage of egg mosaics from the in vivo efficiency assay (Spearman correlation p < 0.01). (B) Percentage of eggs mosaicism and (C) mutagenesis efficiency is not correlated to the in silico prediction by the CRISPOR software (Spearman correlation p > 0.05).