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. 2019 Jul 15;10:891. doi: 10.3389/fphys.2019.00891

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Copyright © 2019 Kotwica-Rolinska, Chodakova, Chvalova, Kristofova, Fenclova, Provaznik, Bertolutti, Wu and Dolezel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Images of polyacrylamide gels showing heteroduplex mobility assay performed on antenna and gonads of the wild type (WT) and G₀ bugs (G₀ bug 1–3). Eggs were injected simultaneously with the tim 1587 and tim 2114 sgRNAs. The same tissue was screened for mosaicism at the 1587 and 2114 loci. The lack of mosaicism in the somatic tissue (antenna) does not indicate the lack of gonadal mosaicism. The occurrence of gonadal mosaicism is a good predictor for the production of mutated progeny. Numbers below pictures indicate the percentage of mutated F₁ progeny to the total progeny of the particular G₀ parent (for detailed numbers see Supplementary Table 2).