TABLE 4.

Comparison between the efficiency of Cas9 mRNA and Cas9 protein in generating heterozygotes in the targeted region of three genes per (short region – perS; perS 1 and perS 4 sgRNA were injected together), pdf (PDF 1) and TEFLamide (TEFL 2).

		No of eggs	No of F1 heterozygotes/
Guide	Cas9 source	injected	No of G0 parents
perS 1	mRNA (400 ng/μl)	145	0/0
perS 1	Protein (250 ng/μl)	180	0/0
perS 1	Protein (500 ng/μl)	165	2/1
perS 4	mRNA (400 ng/μl)	145	0/0
perS 4	Protein (250 ng/μl)	180	1/1
perS 4	Protein (500 ng/μl)	165	0/0
PDF 1	mRNA (400 ng/μl)	170	0/0
PDF 1	Protein (250 ng/μl)	179	1/1
PDF 1	Protein (500 ng/μl)	140	0/0
TEFL 2	mRNA (400 ng/μl)	158	0/0
TEFL 2	Protein (250 ng/μl)	151	0/0
TEFL 2	Protein (500 ng/μl)	120	2/2