TABLE 5.
Comparison between the efficiency of the chimeric single guide RNA (sgRNA) produced by in vitro transcription and commercial crRNA targeting the same sequence in generation of heterozygotes in pdf gene.
| No of eggs | No of F1 heterozygotes/ | |||
| sgRNA | Cas9 source | guide source | injected | No of G0 parents |
| PDF 1 | Protein (250 ng/μl) | sgRNA (200 ng/μl) | 179 | 1/1 |
| PDF 1 | Protein (500 ng/μl) | sgRNA (200 ng/μl) | 140 | 0/0 |
| PDF 1 | Protein (250 ng/μl) | crRNA + tracrRNA (119 ng/μl + 200 ng/μl) | 125 | 0/0 |
| PDF 1 | Protein (500 ng/m) | crRNA + tracrRNA (119 ng/μl + 200 ng/μl) | 130 | 2/2 |
| PDF 1 | Protein (250 ng/μl) | crRNA + tracrRNA (238 ng/μl + 400 ng/μl) | 106 | 0/0 |
| PDF 1 | Protein (500 ng/μl) | crRNA + tracrRNA (238 ng/μl + 400 ng/μl) | 143 | 3/3 |