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Comparison of number of heterozygotes found in F1 generation when young (2–4 h AEL) and mixed stages of eggs (0–12 h AEL) were injected with the identical CRISPR/Cas9 mixture targeting the same sequence of the pdf gene.
| Injections | No of eggs | No of F1 heterozygotes/ | |
| Guide | AEL (h) | injected | No of G0 parents |
| PDF1 crRNA + tracrRNA | 2–4 | 130 | 2/2 |
| PDF1 crRNA + tracrRNA | 0–12 | 87 | 21/4 |
