TABLE 8.
Percentage of gonadal mosaicism in G0 bugs injected with selected sgRNAs and the efficiency of the transfer of the genome modifications to the F1 progeny (for details refer to Supplementary Table 2).
| No of G0 | No of G0 | No of G0 gonadal | % of F1 | |
| fertile | gonadal | mosaics producing | heterozygotes | |
| Guide | adults | mosaics (%) | F1 heterozygotes (%) | in F1 progeny |
| tim 1587 | 48 | 23 (47.9) | 16 (69.6) | 4.2–66.7 |
| tim 2114 | 48 | 11 (22.9) | 6 (45.4) | 5.0 – 26.7 |
| C-cry2 194 | 14 | 5 (35.7) | 5 (100) | 1.4 – 7.4 |
| perSLIH 3 | 10 | 2 (20.0) | 0 | – |
| perSLIH 4 | 4 | 2 (50.0) | 2 (100) | 8.3 – 22.5 |
| perL 2 | 7 | 3 (42.8) | 3 (100) | 9.1 – 60.0 |
| perL 3 | 12 | 7 (58.3) | 7 (100) | 5.0 – 61.5 |
| PDF 1 crRNA | 6 | 4 (66.6) | 4 (100) | 23.9 – 33.3 |
| PDF 2 | 5 | 5 (100) | 5 (100) | 10.0 – 77.3 |
| TEFL 3 | 9 | 6 (66.6) | 6 (100) | 9.1 – 35.7 |
| TEFL 5 | 10 | 2 (20.0) | 1 (50.0) | 2.7 |